If you are using an E2B USB drive larger than 128GiB and have updated it, the \grldr file may now be outside the 128GiB limit.
Some buggy BIOSes have a USB drive bug and cannot access sectors past 128GiB on a USB drive, that is why I strongly recommend the first partition (and second) are within 128GiB of the start of the disk.
The solution is to remake your USB drive using a smaller partition 1 (below 128GiB) and then copy all your payload files back on (or don’t use systems with buggy BIOSes).
If Partition 1 is less than 128GiB then you may have a corrupt NTFS filesystem or the \grldr file may be missing. First fix the filesystem (e.g. using chkdsk U: /f where U: is E2B partition 1) and then copy all the files from the E2B download back onto the E2B USB drive (overwriting any existing files – your payload files and MyE2B.cfg file will not be changed).